Journal: PLoS ONE

Article Title: Visualization of X4- and R5-Tropic HIV-1 Viruses Expressing Fluorescent Proteins in Human Endometrial Cells: Application to Tropism Study

doi: 10.1371/journal.pone.0169453

Figure Lengend Snippet: The two right columns correspond to untreated cells. The two central columns correspond to the addition of a mixture of Il-1β (25 ng/ml) and TNF-α (10 ng/ml) pro-inflammatory cytokines 24 h post-infection. The two right columns correspond to the addition of azydothimidine (AZT) 10 μM 2 h before infection. Results are representative of 6 independent experiments; each of them was performed in duplicate as well as the measurement of total HIV DNA.

Article Snippet: In some experiments, cells infected with HIV for 24 h were incubated with proinflammatory cytokines rhTNF-α (10 ng/ml) and rhIL-1β (25 ng/ml) (Peprotech) for an additional 24 h, before quantifying intracellular and extracellular HIV RNA and DNA as well as p24 production in cell supernatant.

Techniques: Infection

Journal: JCI Insight

Article Title: CC16 augmentation reduces exaggerated COPD-like disease in Cc16 -deficient mice

doi: 10.1172/jci.insight.130771

Figure Lengend Snippet: In A , WT and Cc16 –/– mice were exposed to air (6 mice/group) or CS for 8 weeks (6–7 mice/group), and CS-exposed mice were treated thrice weekly with rhCC16 (75 μg of rhCC16) or vehicle. NF-κB that translocated to the nucleus was quantified in nuclear extracts of whole lung samples using a TransAM NF-κB kit. In B , NF-κB luciferase reporter A549 cells were grown to at least 80% confluence and preincubated for 3 hours with 100 μg/mL of rhCC16. Cells were then activated with rhTNF-α (1–10 ng/mL), and 8 hours later, the cells were lysed and luciferase activity was measured. In C , WT and Cc16 –/– mice were exposed to air (3–5 mice/group) or CS for 12 weeks (10–12 mice/group), and CS-exposed mice were treated once daily with a solution of IMD0354 (6 mg/kg body weight) or vehicle via the intraperitoneal route during the last 6 weeks of the CS exposures (5–6 mice/group). BAL was performed, and BAL total leukocytes ( C ), macrophages ( D ), and PMNs ( E ) were counted. Boxes in the box plots show the medians and 25th and 75th percentiles, and whiskers show the 10th and 90th percentiles. Data were analyzed using 1-way ANOVAs followed by pairwise testing with Mann-Whitney U tests. In A and C – E , asterisks indicate P < 0.05 vs. air-exposed mice belonging to the same genotype or the group indicated; in B , asterisks indicate P < 0.05 vs. negative control in lane 1 or the group indicated.

Article Snippet: NF-κB luciferase reporter A549 cells (Lonza) ( ) were preincubated with 100 μg of rhCC16 for 3 hours, followed by either CSE at 1%–10% or 1–10 ng/mL rhTNF-α (Peprotech).

Techniques: Luciferase, Activity Assay, MANN-WHITNEY, Negative Control